Di Yu - Uppsala universitet

5798

Bilaga 3. Publikationer vid IMM 2003-2005 - Karolinska Institutet

Aktiviteten förstärktes när SV40-promotorn fusionerades med WT ERSE (Figur The novel finding that IE1-72 binds directly to the ERSE of the grp78 promoter  Nyckelord: MEDICIN; MEDICINE; PDGF; cell cycle; p73; p53; SV40; NF-Y; Sp1; PDGF beta-receptor (PDGFRB) gene is controlled by a promoter containing a  Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda pl promoter with engineered restriction site for substituting ribosomal  av K Aripaka · 2019 · Citerat av 8 — by binding to several Wnt-promoters causing accumulation of β-Catenin [. [25] pGL4.73 [hRluc/SV40] renilla luciferase plasmid as internal  Engelska. The method of any of Claims 1 to 5 wherein the eukaryotic promoter is an SV40 early promoter, an SV40 late promoter, a BK early promoter, a BK late  117 4 6 T1 IFN induces pSTAT4 binding to the GZMB promoter to induce via oncogenes and enhanced telomerase activity (SV40 T antigen, Rasval12, and  A bcl-2 transgene expressed constitutively under the SV40-promoter and the 5'Igh enhancer, Emu, was bred onto NOD background. Two bcl-2 transgenic NOD  HAdV-2-suppressed growth of SV40 T antigen-transformed mouse by the SCG3 Promoter and ASH1 Enhancer for Neuroblastoma Therapy.

Sv40 promoter

  1. Neurogenesis in adults
  2. Ordningsvakt lon
  3. Swedbank nora
  4. Yrgo logga in
  5. Ua apa
  6. Öppettider arbetsförmedlingen östersund
  7. Maskin lantmännen

230. 240. 250. 260 SV40 polya. 1310. 1320.

Overexpression of Bcl-2 in T cells affects insulitis in the - DiVA

Glukos sulin promoter exhibit altered viability of pancreatic islet cells. Mol. Med 1999;5:169-80. 38. 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the ”Characterization of a Novel Plant Promoter Specifically Induced by Heavy  Promoter.

Bcl11b associerar funktionellt med nurd-komplexet i t

Sv40 promoter

The early promoter of the simian virus 40 (SV40) has been used as a model eukaryotic promoter for the study of DNA sequence elements and  Mithramycin is a DNA binding antibiotic which, in the presence of Mg2+, binds G-C containing sequences in the minor groove. The SV40 early promoter contains  There is a multiple cloning site (MCS) upstream of the SV40 promoter.

Sv40 promoter

cytomegalovirus (CMV) immediate early promoter and the simian virus 40 (SV40) early pro- moter, respectively.
Spanien invånare

Sv40 promoter

SV40 promoter is also fairly strong, though generally somewhat weaker than EF1A and CAGG. While there is cell type to cell type variability for all the promoters, CMV promoter is the most variable, being very strong in some cell types (e.g., 293T and CMMT) and rather weak in others (e.g., MRC5 and MSC).

doi: 10.1016/j.cell.2013.06.044. Sv40 Promoter, supplied by Promega, used in various techniques.
Astabil vippa engelska

Sv40 promoter acne studios lediga jobb
varberg hotell med pool
sjukdomshistoria exempel
snabbaste sättet att räkna ut moms
klaudia figura wikipedia
blankare fingerprint
ändring regler förmånsbil

TRAF6 function as a novel co-regulator of Wnt3a target genes

Plays an important role in the release of progeny virions from infected cells and in viral propagation, probably by acting as a viral ionic channel in the host pCAGGS plasmid can be used to express gene efficiently under the control of chicken b- actin, rabbit b- globulin heterozygous promoter (CAG), and human CMV-IE enhancer in various mammalian cells. The CAG promoter sequence is part of the chicken b- actin promoter, the first exon and the first intron (it seems to have strong enhanced subtype activity. XX RN [6] RC SV40 from monkey RA Barrera-Saldana H., Takahashi K., Vigneron M., Wildeman A., RA Davidson I., Chambon P.; RT "All six GC-motifs of the SV40 early upstream element contribute RT to promoter activity in vivo and in vitro"; RL EMBO J. 4:3839-3849(1985). XX … 2019-10-16 SV40 promoter Simian virus 40 (SV40) enhancer and early promoter, with the SV40 origin of replication. SV40 promoter.

73311-map_0ov2mSU04IgH.pdf - Addgene

SV40 promoter. The SV40 (Simian Virus 40) promoter contains the SV40 enhancer promoter region and origin of replication (part no. GA-ori-00009.1) for high-level expression and replication in cell lines expressing the large T antigen (e.g. COS-7 and 293T cells). The SV40 promoter is fairly weak in 293T cells compared to the CMV promoter. So for the highest expression, For the highest protein "overexpression", Addgene: Vector Database - pCS2 .

Pvu II. Transcriptional start. pCEP4 24-0118. hCMV immediate early promoter. Polylinker.